Cytophotometry for succinate dehydrogenase activity in relation to tissue protein content with tetranitro blue tetrazolium (TNBT).
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Tetranitro-blue Tetrazolium Reduction in Proteus Vulgaris
In Bacillus subtilis the reduction of potassium tellurite and of tetranitro-blue tetrazolium (TNBT) give comparable results: reduced products appear deposited in particular membranous organelles. For this reason these specialized organelles are considered to be chondrioids (1-3). In the Gram-negative Proteus vulgaris similar membranous structures were never observed by us (4, 5). Therefore it i...
متن کاملTetranitro-blue Tetrazolium Reduction in Bacillus Subtilis
In order to decide whether in the bacterial cell the enzymes of the respiratory chain are located in "particulate elements" (1) or in the plasma membrane (2) we investigated the reduction of potassium tellurite in Gram-positive and Gram-negative bacteria. For the first time in 1962 (3), and in more detail in 1964 (4), we reported that with Bacillus subtilis we had found that reduction products ...
متن کاملSuccinate Dehydrogenase Activity Assay in situ with Blue Tetrazolium Salt in Crabtree-Positive Saccharomyces cerevisiae Strain
A spectrophotometric method for determining succinate dehydrogenase (SDH) activity assay in azide-sensitive yeast Saccharomyces cerevisiae has been developed. The permeabilization of yeast cells by 0.05 % digitonin permitted to study yeast enzymatic activity in situ. The reduction of blue tetrazolium salt (BT) to blue tetrazolium formazan (BTf) was conducted in the presence of phenazine methosu...
متن کاملThe Use of Tetranitro-blue Tetrazolium for the Cytochemical Localization of Succinic Dehydrogenase
Succinic dehydrogenase (SDH) was localized in the mitochondria of Sarcoma 37 ascites tumor cells by the use of tetranitro-BT (TNBT) and nitro-BT (NBT) in smear preparations. Results with each tetrazolium salt as electron acceptor were evaluated with respect to: (a) size and shape of the formazan precipitate relative to standard mitochondrial morphology; (b) crystallization phenomena of reduced ...
متن کاملThe Demonstration of the Succinic Dehydrogenase System in Bacillus Subtilis Using Tetranitro-blue Tetrazolium Combined with Techniques of Electron Microscopy
Activity of the succinic dehydrogenase system was studied in Bacillus subtilis utilizing combined techniques of cytochemistry and electron microscopy. Organisms were incubated in a medium containing tetranitro-blue tetrazolium (TNBT) which served as an electron acceptor. Enzymatic activity, as evidenced by deposition of TNBT-formazan, was found on membranous organelles associated with the cytop...
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1986
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.19.241